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Medchemexpress LLC 5-Bromo-3-indolyl β-D-galactopyranoside | 97753-82-7 | 99.7% | 374.18 | 250 MG
Supplier: Medchemexpress LLC 250MGHY137276
5-Bromo-3-indolyl β-D-galactopyranoside (Bluo-Gal) is a chromogenic substrate for β-galactosidase. It is hydrolyzed by the enzyme to generate a 5-bromoindole intermediate, which then oxidizes to form an insoluble blue precipitate. It specifically recognizes bacterial β-galactosidases, such as the product of the *Escherichia coli lacZ* gene, and reacts at pH 7.4. This makes it suitable for light and electron microscopic observations and for histochemical detection of reporter gene expression in transgenic organisms.
- Suitable for localization analysis of β-galactosidase activity in mouse embryos or muscle tissues.
- The 5-bromoindigo precipitate is a birefringent needle-shaped crystal.
- Easier to identify under a low-power microscope than X-Gal.
- Resistant to elution by organic solvents.
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