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Zymo Research Corporation 5-Fluoroorotic Acid (5-FOA), 5g
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Supplier:  Zymo Research Corporation F90015

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Curing yeast strains of plasmids, plasmid shuffle, allelic replacement, and two-hybrid screens are standard methods employing 5-FOA (Boeke, J.D. et al. 1984 and 1987; Vidal, M. 1997). The nontoxic 5-FOA compound is converted to toxic 5-flurouracil in yeast strains expressing a functional URA3 gene (orotine-5’- monophosphate decarboxylase), whose normal cellular function is in the synthesis of uracil. Practically, strains that are phenotypically Ura+ become Uraand 5-FOAR (resistant), after selection.

Catalog No. NC9258228


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