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ACROBiosystems Recombinant Protein;Human VEGF165 Protein, His Tag;HEK293;50UG;VE5,RP1-261G23.1,MGC70609,MVCD1,MVCD-1,VEGF 165,VEGF-A,VEGFA,VPF,VEGF165,VEGF-165,VEGF121,VEGF120,VEGF186,VEGF164,VEGF110
Supplier: ACROBiosystems VE5H524850UG
Recombinant Protein;Human VEGF165 Protein, His Tag;50UG;Human VEGF165, His Tag (VE5-H5248) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 191 (Accession # P15692-4).;This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 21.1 kDa. The protein migrates as 24 kDa and 25-27 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Human VEGF R1&VEGF R2, Fc Tag at 2 μg/mL (100 μL/well) can bind Human VEGF165, His Tag (Cat. No. VE5-H5248) with a linear range of 0.2-4 ng/mL (QC tested).;Avastin (Bevacizumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human VEGF165 Protein, His Tag (Cat. No. VE5-H5248) with an affinity constant of 1.03 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
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