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ACROBiosystems Recombinant Protein;Human CD19 (20-291) Protein, Fc Tag, low endotoxin;HEK293;100UG;CD9,CD19,CD-19,B4,B-4,CVID3,CVID-3,MGC12802
Supplier: ACROBiosystems CD9H5259100UG

Recombinant Protein;Human CD19 (20-291) Protein, Fc Tag, low endotoxin;100UG;Human CD19 (20-291), Fc Tag, low endotoxin (CD9-H5259) is expressed from human 293 cells (HEK293). It contains AA Pro 20 - Lys 291 (Accession # AAH06338).;This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 56.3 kDa. The protein migrates as 56-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized FMC63 at 1 μg/mL (100 μL/well) can bind Human CD19 (20-291), Fc Tag, low endotoxin (Cat. No. CD9-H5259) with a linear range of 0.039-0.625 μg/mL (QC tested).;293 cells were transfected with FCM63-scFv and RFP tag. 2x105 of the cells were first incubated with A. Human Fc tag control. B. Recombinant human CD19, Fc Tag, low endotoxin (Cat. No. CD9-H5259, 10 μg/ml ). C. Recombinant human CD19, Fc Tag, low endotoxin (Cat. No. CD9-H5259, 10 μg/ml ) and FMC63 (Mouse anti-CD19 antibody). The FITC anti-human IgG Fc was used to analyse with FACS. RFP was used
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