AdipoGen Acridine Orange hemi(zinc chloride) (100 g)

Supplier:  AdipoGen CDXA0668G100

Chemical. CAS: 10127-02-3. Formula: C17H20ClN3 . HCl . 1/2ZnCl2. MW: 369.96. Acridine Orange hemi(zinc chloride) salt is a cell-permeable metachromatic fluorescent dye that stains DNA and RNA. It is used as a nucleic acid-selective fluorescent cationic dye useful for cell cycle determination. Being cell-permeable, it interacts with DNA and RNA by intercalation or electrostatic attractions respectively. When bound to DNA, it is very similar spectrally to fluorescein, with an excitation maximum at 502nm and an emission maximum at 525nm (green). When acridine orange associates with RNA, the excitation maximum shifts to 460nm (blue), and the emission maximum shifts to 650nm (red). Acridine orange will also enter acidic compartments such as lysosomes where it becomes protonated and sequestered. Within these low pH vesicles, the dye emits red fluorescence when excited by blue light.

Catalog No. 50-205-4293