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Biotium 10-Acetyl-3, 7-dihydroxyphenoxazine
Supplier: Biotium 10061
10-Acetyl-3,7-dihydroxyphenoxazine (N-Acetyl-3,7-dihydroxyphenoxazine or ADHP), marketed under the name Amplex Red, is regarded as the best fluorogenic substrate for peroxidase because it is highly specific and stable. The substrate itself is nearly colorless and nonfluorescent until it is oxidized by H2O2 (reacting in a 1:1 stoichiometry) in the presence of horseradish peroxidase (HRP) to become the highly red fluorescent resorufin. H2O2 is produced by many enzymatic reactions, and therefore ADHP can be used to detect the activity of many different enzymes. It can be used to detect HRP in immunoassays or detect trace amounts of hydrogen peroxide (H2O2) using fluorometer or microplate reader.Properties: Ex/Em (pH 9)= 571/585 nm (end product); e= 54,000 (end product); White to off-white solid soluble in DMSO or DMF; Store desiccated at -20°C and protect from light; C14H11CNO4; MW: 257.24; [119171-73-2]Amplex Red is a registered trademark of Thermo Fisher Scientific.
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