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ABclonal Technology HRP-conjugated Mouse anti DDDDK-Tag mAb
Supplier: ABclonal Technology AE0241000UL
The FLAG-tag (DYKDDDDK) is a short polypeptide added to proteins via recombinant DNA technology. It is widely used for protein purification by affinity chromatography and for studying proteins in living cells. The tag helps distinguish recombinant proteins from native ones and enables isolation of multi-subunit complexes due to its gentle purification process. It also allows production of proteins pure enough for structural studies like x-ray crystallography. The FLAG-tag can be detected using specific antibodies, making it useful in assays such as immunofluorescence, SDS-PAGE, and Western blotting, especially when no antibody exists for the target protein.
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