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ATCC Agarabi; Ovary; Chinese Hamster (Cricetulus griseus)
Supplier: ATCC CRL3440
Agarabi CHO is a cell line exhibiting lymphoblast and epithelial (per depositor) morphology that were isolated from the ovaries of a Chinese hamster (Cricetulus griseus). The cells were stably transfected with a two-vector system expressing a model mouse-human chimeric IgG1 antibody. Single cell derived clones were isolated by limiting dilution and expanded. Agarabi CHO was subjected to further amplification by treatment with 2 μM methotrexate for up to 42 days and frozen. On thaw, Agarabi CHO was serially subcultured in the absence of methotrexate on a 3-4 day regime for ~2-3 weeks and cryopreserved. This cell line is an industrially-relevant research material that can be used in the study of continuous biomanufacturing, process analytical technology, and bioprocess design.
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