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ATCC ATCC CA-HPV-10 (CRL-2220) Human Prostate Adenocarcinoma Cell Line
Supplier: ATCC CRL2220
CA-HPV-10 (CRL-2220) is a human prostate adenocarcinoma epithelial cell line immortalized by transfection with HPV18 DNA. The line expresses keratins 5 and 8, the HPV E6 oncoprotein, and prostate-specific antigen (KLK3). Intended for in vitro research use only.
- Tissue: prostate adenocarcinoma.
- Species: human (Homo sapiens).
- Morphology: epithelial; growth: adherent.
- Immortalization: HPV18 DNA transfection confirmed by PCR.
- Culture conditions: keratinocyte serum-free medium supplemented with bovine pituitary extract and recombinant EGF; 37°C; 5% CO2.
- Biosafety: BSL-2; cells contain HPV sequences.
- Authentication: mycoplasma not detected; STR profile provided.
- Storage: vapor phase liquid nitrogen; do not store at -70°C.
- Restrictions: commercial use requires a license; material transfer agreement applies.
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