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Rockland Immunochemicals BCIP/NBT MEMBRANE ALKALINE PHOSPHATASE SUBSTRATE - NBT-100

Supplier: Rockland Immunochemicals NBT100
BCIP/NBT Membrane Alkaline Phosphatase Substrate is an artificially manufactured chromogenic substrate for use in sensitive colorimetric assays. Alkaline phosphatase hydrolyzes the BCIP to 5-bromo-4-chloro-3-indole, which is oxidized by oxygen in the atmosphere, yielding a blue dye. In the presence of NBT, an insoluble dark blue precipitate is formed after a reduction reaction instead.
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