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IBA LifeSciences Biotin, 2 g
Manufacturer: IBA LifeSciences 21016002
Biotin is a water-soluble B-complex vitamin (vitamin B7 or vitamin H)It binds very tightly (irreversibly) to Streptavidin, Strep-Tactin and Strep-TactinXT. Therefore biotin allows the efficient elution of Strep-tagged proteins from Strep-TactinXT, MagStrep """"""""""""""""type3"""""""""""""""" XT beads and Strep-Tactin Spin columns. Biotin binds irreversible to Strep-Tactin, therefore the resin cannot be regenerated. Strep-TactinXT can be regenerated after elution with biotin by addition of 10mM NaOH (freshly prepared).
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