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ACROBiosystems ACROBiosystems Biotinylated SARS-CoV-2 Spike Trimer (T19R, G142D, EF156-157del, R158G, L452R, T478K, D614G, P681R, D950N), His,Avitag™ (MALS verified)

Supplier:  ACROBiosystems SPNC82EC25UG

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Recombinant Protein;25UG;Biotinylated SARS-CoV-2 Spike Trimer, His,Avitag (SPN-C82Ec) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1). The mutations (T19R, G142D, EF156-157del, R158G, L452R, T478K, D614G, P681R, D950N) were identified in the SARS-CoV-2 variants which emerged in India (known as B.1.617.2). Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively. ;This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 139.5 kDa. The protein migrates as 160-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Biotinylated SARS-CoV-2 Spike Trimer, His,Avitag (Cat. No. SPN-C82Ec) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) p

Catalog No. 50-210-6993


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