Sigma Aldrich Fine Chemicals Biosciences BioTracker 640 Red C2fM4 6

Supplier:  Sigma Aldrich Fine Chemicals Biosciences SCT127

BioTracker synaptic dyes are a series of fluorescent cationic styryl dyes developed to follow synaptic activities at neuromuscular junctions or synapses. These dyes typically have a lipophilic tail at one end and a highly hydrophilic at the other end. In the presence of cells or tissue preparations the dyes partition between the aqueous phase where the dyes are virtually non-fluorescent and the outer leaflet of the cell surface membranes where the dyes insert the lipophilic end into the membranes and become intensely fluorescent. During endocytosis following nerve stimulation the dyes become trapped inside the vesicles. Thus after washing off the dyes on the cell surface the fluorescent signal is proportional to the number of newly formed vesicles. On the other hand during exocytosis the dyes are released from the vesicles along with neurotransmitters causing a decrease in fluorescent signal. As a result the change in fluorescent intensity reflects the amount of endocytosis/

Catalog No. 50-290-6612