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Medchemexpress LLC BirA protein, E. coli (His) | >90.0% | 50UG
Supplier: Medchemexpress LLC HYP70215450UG
BirA is a recombinant biotin ligase produced in E. coli with an N-terminal 6xHis tag. It activates biotin in the presence of ATP to form the biotinyl-5'-adenylate complex and is supplied as a stabilized, filtered solution for enzymatic applications and biochemical assays.
- Purity greater than 90% as determined by reducing SDS-PAGE.
- Predicted molecular weight ~36.6 kDa by SDS-PAGE.
- Formulated in 50 mM Tris-HCl (pH 7.5), 200 mM NaCl, 20% glycerol, 1 mM DTT.
- Endotoxin level less than 1 EU/μg (LAL method).
- Recommended storage at -80°C; avoid repeated freeze-thaw cycles.
- Available in a 50 μg solution size suitable for assay use.
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