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ILEX LIFE SCIENCES LLC Biocolor Blyscan Glycosaminoglycan Dye and Dye Dissociation Reagents Assay Components Pack 220 ML 1/EA
Supplier: ILEX LIFE SCIENCES LLC B1500
Biocolor Blyscan Glycosaminoglycan (sGAG) Dye Dissociation Reagents Component Pack (2 x 2 x 110 ml) Includes 2 x 110 ml Dye Reagent 2 x 110 ml Dissociation Reagent The Blyscan Glycosaminoglycan Assay is a quantitative colorimetric dye-binding method for the analysis of sulfated proteoglycans and glycosaminoglycans (sGAG) It can be used to measure the total sGAG content and can also be used to determine the O- and N- sGAG ratio within test samples The assay uses 1 9-dimethylmethylene blue dye label which is employed under conditions that provide a specific label for the sulfated polysaccharide component of proteoglycans or the protein free sGAG chains Applications Cell culture derived material soluble sGAGs from fluids (ex synovial fluid urine) fibrous and hyaline cartilages tissues (ex arteries heart lung skin tumors) Soluble sGAGs may be assayed directly solid materials will require a preliminary papain extraction Sensitivity 0 5 g Assay run time 1 hr
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