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Enzo Life Sciences Calcineurin (human), (recombinant) (5000 U)

Supplier: Enzo Life Sciences BMLSE1635000

Alternative name: Protein phosphatase 2B. PP2B. MW: 60 kDa (calcineurin Aα) + 15 kDa (calcineurin B). Concentration: 100 U/µl. Purity: ≥90% (SDS-PAGE). Formulation: Liquid. In 1X KI-128 Assay Buffer with increased DTT. Concentration (50mM TRIS, pH 7.5, containing 100mM sodium chloride, 6mM MgCl2, 5mM DTT, 0.025% NP-40 and 0.5mM CaCl2). Source: Produced in E. coli. Human calcineurin Aα (MW 60kDa) and calcineurin B (MW 15kDa) heterodimer. Both subunits are coexpressed in a construct with yeast myristoyl-CoA:protein N-myristoyltransferase. The resulting highly active calcineurin (protein phosphatase 2B) is N-myristoylated on the CaNB subunit, similar to the native protein. UniProt: Q08209 (subunit Aα), P63098 (subunit Bα). Handling: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C. Long Term Storage: -80°C
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