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Enzo Life Sciences Cathepsin D & E substrate (fluorogenic) (1 mg)
SDP

Supplier:  Enzo Life Sciences BMLP1450001

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Quenched fluorogenic substrate for cathepsin D (kcat/Km=1.09 x 10 M-1s-1) and cathepsin E (kcat/Km=1.56 x 10 M-1s-1). Mca fluorescence is quenched by the Dnp group until cleavage separates them. Activity of the two enzymes on this substrate can be distinguished by the use of Ascaris pepsin inhibitor, which inhibits cathepsin E, but not cathepsin D. This substrate is also cleaved by MMP-2, MMP-3, MMP-9, but not MMP-1 nor MMP-13. This substrate is useful for inhibitor screening and kinetic analysis. Ex.: 320-340 nm, Em.: 393-420 nm. Mca-Pro-Leu-OH (BML-P127) can be used to calibrate for Mca fluorescence. MW: 1756.0. Formulation: Trifluroacetate salt. Purity: ≥95% (HPLC). Sequence Mca-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH2 [Mca= (7-methoxycoumarin-4-yl)acetyl; Dnp=dinitrophenyl]. Appearance: Yellow powder. Solubility: Soluble in DMSO (1mM) or 50% acetonitrile (10mg/ml). Long Term Storage: -20°C. Handling: Protect from light. Keep cool and dry.

Catalog No. NC1421631


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