Apexbio Technology LLC Cell lysis buffer for WB and IP(Synonyms: Cell extraction buffer, Cell lysis solution, Tissue lysis buffer, Protein extraction reagent), 100mL.

Supplier:  Apexbio Technology LLC K1123100

Cell lysis buffer for WB and IP is a solution widely utilized for rapid lysis of cells and tissues under non-denaturing conditions to release intracellular proteins while preserving protein-protein interactions The buffer contains 20 mM Tris (pH 7 5) 150 mM NaCl and 1% Triton X-100 supplemented with sodium pyrophosphate -glycerophosphate EDTA sodium orthovanadate (Na VO ) and protease inhibitor leupeptin to prevent proteolysis and dephosphorylation Resulting protein extracts are suitable for downstream analyses including Western blotting immunoprecipitation co-immunoprecipitation ELISA and PAGE assays across animal plant fungal or bacterial samples

Catalog No. 50-000-61376