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Sigma Aldrich Fine Chemicals Biosciences Con A SepharoseR 4B Cytiva
Supplier: Sigma Aldrich Fine Chemicals Biosciences GE17044001
Con A Sepharose(R) 4B with immobilized Concanavalin A is an affinity chromatography media for capture of glycosylated biomolecules including glycoproteins and glycolipids. Stronger affinity for alpha-D-glucose than alpha-D-mannose.Con A Sepharose(R) is Concanavalin A coupled to Sepharose(R) 4B by the cyanogen bromide method. Concanavalin A (Con A) is a tetrameric metalloprotein isolated from Canavalia ensiformis (jack bean). Con A binds molcules containing alpha-D-mannopyranosyl alpha-D-glucopyranosyl and sterically related residues. The binding sugar requires the presence of C-3 C-4 and C-5 hydroxyl groups for reaction with Con A. Con A coupled to Sepharose(R) is routinely used for separation and purification of glycoproteins polysaccharides and glycolipids.
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