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Electron Microscopy Sciences CryoCapsule High Pressure Freezing
Supplier: Electron Microscopy Sciences 3750
The CryoCapsule is used in High Pressure Freezing (HPF) and correlative light and electron microscopy (CLEM). Comparable to a small petri dish, it is composed of a landmarked sapphire disc and a gold spacer ring (50um thick) maintained together by a plastic ring. The specimens are encapsulated between the support sapphire disc (carbon landmarked) and a covering sapphire disc.
The CryoCapsule is loaded into a specific adaptor (HPM010, HPM100, HPF compact 02). Live cell imaging is done directly on the specimen in the CryoCapsule prior to HPF. Post-HPF, the specimen is processed for freeze substitution and room temperature sectioning.
CryoCapCell has developed a set of tools to manipulate the CryoCapsule in most scientific environments.
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