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Dojindo Molecular Technologies Inc DALGreen – Autophagy Detection (20 nmol)
Supplier: Dojindo Molecular Technologies Inc D67510
DALGreen is used to detect autophagy in live cells. Autophagy is an intracellular degradation system, where dysfunctional proteins and organelles are degraded. In this process, aggregated dysfunctional proteins are surrounded by the double membrane to form an autophagosome. DALGreen, which is a small hydrophobic molecule, passes the plasma membrane of live cells and is incorporated in the autophagosome. After lysosomes fuse with the autophagosome, the environment in the autolysosome becomes acidic. DALGreen fluoresces stronger as acidity increases. The quality of this dye enables live-cell imaging with fluorescence microscopy and quantitative assay by flow cytometry. Excitation and emission spectra of DALGreen: (λex: 405 nm, λem: 525 nm). For monitoring autophagosomes, we recommend DAPGreen [D676] and DAPRed [D677]. Dojindo offers various reagents for autophagy research. (https://www.dojindo.com/autophagy-research/#jmp-b).
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