KRISHGEN BIOSYSTEMS Robust and sensitive Immunoassay for the Qualitative / semi-quantitative screening of double stranded RNA in mRNA based preparations. Assay Range: 0.781 – 100 ng/ml; Sensitivity: 3 ng/ml.

Supplier:  KRISHGEN BIOSYSTEMS KBBA56

Pack Size: 5 x 96 wells. Introduction: The J2 anti-dsRNA IgG2a monoclonal antibody (Schonborn et al. 1991) has become the gold standard in dsRNA detection. It was used initially for the study of plant viruses, but since the seminal paper of Weber et al. in 2006, where J2 was used to show that all the positive strand RNA viruses tested produced copious amounts of dsRNA in infected cells, this antibody has been used extensively in a wide range of systems, as documented in over 200 scientific publications. J2 can be used to detect dsRNA intermediates of viruses as diverse as Hepatitis C virus, Dengue virus, rhinovirus, Chikungunya virus, Rabies virus, Polio virus, Classic swine fever virus, Brome mosaic virus and many more in cultured cells and also in fixed paraffin-embedded histological samples. This double-stranded dsRNA ELISA is pre-coated and validated against a standard, allowing for faster, consistent analysis of dsRNA contaminants.

Catalog No. NC2747133