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Enzo Life Sciences FLUOR DE LYS developer I concentrate (300 µl)
Supplier: Enzo Life Sciences BMLKI1050300
An essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS substrate. FLUOR DE LYS Developer is an essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS substrate. An appropriate FLUOR DE LYS family substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the FLUOR DE LYS Developer produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1x Developer dilution (e.g. Trichostatin A) for class I and II HDACs, nicotinamide for sirtuins). Shipping: Shipped on Blue Ice. Long Term Storage: -80°C
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