Sigma Aldrich Fine Chemicals Biosciences Gecko2 Human Whole Genome1EA

Supplier:  Sigma Aldrich Fine Chemicals Biosciences HGECKO2A1EA

Developed by Feng Zhangs lab at the Broad Institute the human GeCKO v2 (all-in-one vector) libraries consist of over 100000 unique gRNAs for gene knock-out in the human genome. Using an all-in-one lenti CRISPR vector which co-expresses Cas9 gRNA with a puromycin selection marker the version 2 vector produces nearly 10X higher titer virus than version 1. The human single vector system GeCKO pool is provided in 8 x 25 ul aliquots at a minimum titer of 5X108 TU/ml (measured by a p24 assay). The GeCKO human library is delivered as two half-libraries (A and B). It is recommended to screen both A and B libraries together which will include 6 sgRNAs per gene (3 sgRNAs in each library). Both libraries contain 1000 non-targeting control sgRNAs. The A library also targets miRNAs (4 sgRNAs per miRNA).

Catalog No. 50-290-9085