ATCC GP+E-86 (CRL-3620) Mouse embryo fibroblast cell line, retroviral packaging and transfection host

Supplier:  ATCC CRL3620

A mouse embryo fibroblast cell line engineered to package ecotropic retroviral genomes and serve as a transfection host for production of retroviral vectors for delivery of foreign genes into susceptible eukaryotic cells. Derived from NIH 3T3 cells via electroporation of plasmids encoding gag/pol and env, it is commonly used for retroviral vector production and transfection workflows.

• Suitable for packaging psi-containing nucleic acids into ecotropic retrovirus genomes.
• Adherent growth with fibroblast morphology.
• Recommended culture at 37°C in 95% air, 5% CO2.
• Complete medium: DMEM supplemented with hypoxanthine (0.015 mg/mL), xanthine (0.25 mg/mL), mycophenolic acid (0.025 mg/mL), and 90% calf serum.
• Subculture ratio typically 1:5 to 1:10; renew medium twice weekly.
• Store cryogenically in vapor phase of liquid nitrogen; cryopreserve in medium with 5% DMSO.

Catalog No. NC3845706