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Enzo Life Sciences HDAC3 / NCOR1 complex (human), (recombinant) (50 µg)
Supplier: Enzo Life Sciences BMLSE5150050
Approximately 1:1 stoichiometric complex of recombinant human HDAC3 (histone deacetylase 3) with a GST fusion protein comprising the Deacetylase Activation Domain (DAD) of human NCOR1 (amino acids 397-503; SANT1 domain plus amino flanking residues). Produced by coexpression of HDAC3 with a GST-NCOR1(DAD) construct in an insect cell system, the complex displays ~100-fold higher deacetylase activity than isolated HDAC3 (based on equal weights HDAC3 assayed at a saturating concentration (100µM) of a fluorogenic peptide substrate p53 379-382, K(Ac)382; BML-KI177).This HDAC3 preparation is active with various fluorogenic peptide substrates, including FLUOR DE LYS Substrate (BML-KI104) and FLUOR DE LYS-SIRT1 (p53 379-382, K(Ac)382; BML-KI177). Purity: ≥90% (SDS-PAGE). Formulation: Liquid. In 50mM TRIS, pH 8.0, 138 mM NaCl and 10% glycerol. Source: Produced in insect cells. Produced in a baculovirus expression system.
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