Thomas Scientific Sporulation Broth, 500 g

Supplier:  Thomas Scientific C979Z83

Sporulation Broth was originally developed by Arret and Kirshbaum for the detection of antibiotic residues in milk and milk products (1). The medium is specified for the preparation of spores, by APHA, for disc assay procedure for the detection of sulfa drugs and antibiotics in milk (2). Peptic digest of animal tissue and beef extract provide nitrogen, sulphur and amino acids and essential trace ingredients. Yeast extract is a rich source of vitamin B; dextrose is the energy source. Suspensions containing large numbers of bacterial spores are obtained using Sporulation Broth. A fresh slant culture of Bacillus subtilis is washed with sterile physiological saline onto the surface of Roux bottles containing 300 ml sterile medium. The bottles are incubated at 35°C for 5 days and the resulting growth is suspended into 50 ml of sterile physiological saline. The growth is washed by centrifuging the suspension and discarding the supernatant. The sediment obtained is re-suspended in fresh s

Catalog No. NC1600473