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Sigma Aldrich Fine Chemicals Biosciences HisTrapTM High Performance
Supplier: Sigma Aldrich Fine Chemicals Biosciences GE17524701
HisTrap(TM) HP columns are prepacked with Ni Sepharose(R) HighPerformance and designed for simple high-reSOLUTIONon purificationof histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).HisTrap(TM) HP 1 mL and 5 mL columns are designed for simple one-step purification of histidine-tagged proteins. The columns are prepacked with Ni Sepharose(R) High Performance which has high binding capacity and low nickel ion leakage that ensures reliable capture of target protein in repeated IMAC purifications.HisTrap(TM) HP columns can also be used for the purification of tagged proteins containing shorter or longer polyhistidine tags such as (histidine)4 or (histidine)10. The shorter (histidine)4 will bind more weakly and the longer (histidine)10 will bind more strongly compared with (histidine)6. This difference in binding strength can be used during purification since (histidine)10 binds more strongly a higher concentration of imidazole can be added to the lysed ce
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