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Innovative Research Inc Hmn PAI1 and tPA Cmplx Kit
Supplier: Innovative Research Inc IHUPAI1TPACXKT
Human PAI-1 and tPA Complex ELISA Kit - This kit is for the quantitative determination of the covalent complex of tPA and its inhibitor PAI-1 in human plasma, serum, urine, cell culture media, or tissue extracts. For RUO. Strip well format. Reagents for up to 96 tests. Human PAI-1 in samples will bind to the monoclonal capture antibody coated on the microtiter plate. Free, latent, and complexed PAI-1 will react with the capture antibody. After washing, polyclonal anti-human tPA primary antibody binds to the captured PAI-1/tPA complex. Excess primary antibody is washed away and bound antibody is then reacted with peroxidase conjugated secondary antibody. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of PAI-1/tPA complex. Color is proportional to the concentration of PAI-1/tPA complex. Free tPA and PAI-1 will not be detected by this assay.
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