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Lonza Walkersville Hu Naive CD8/CD45RA T-Cells
Supplier: Lonza Walkersville 2W302
Na ve CD8 T cells are isolated using a two step isolation process CD8 T Cells are first negatively selected from MNC obtained from leukapharesed blood using an indirect immunomagnetic CD8 labeling system CD45RO microbeads are then used to deplete the CD45RO population leaving a highly enriched CD4 CD45RA Na ve T Cell population Cells are cryopreserved in Lonza Cryo Media and collected in a cryovial Cell purity is determined post thaw using a FACS assay Count and viability is determined using hemacytometer (trypan blue method) Cells are collected from healthy donors following IRB protocols
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