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Sino Biological Human IkB alpha Matched ELISA Antibody Pair Set 15 Plates

Supplier:  Sino Biological SEK1204515

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The Human IkB alpha / NFKBIA ELISA Pair Set is for the quantitative determination of Human IkB alpha / NFKBIA.This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for IkB alpha / NFKBIA coated on a 96-well plate. Standards and samples are added to the wells, and any IkB alpha / NFKBIA present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-IkB alpha / NFKBIA monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of IkB alpha / NFKBIA present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.

Catalog No. 50-007-85098


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