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Innovative Research Inc Human Keratin 16 ELISA Kit, Range: 0.156-10ng/mL, Sensitivity: 0.063ng/mL
Supplier: Innovative Research Inc IHUKRT16KT
Human Keratin 16 ELISA Kit from Innovative Research is intended for the quantitative determination of Human Keratin 16 in biofluid samples, such as serum, plasma, tissue homogenates and other biological fluids. This is a sandwich ELISA in strip well format with reagents for up to 96 tests .KRT16 present in samples reacts with an anti-Human KRT16 capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-KRT16 detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active KRT16 in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Human KRT16.
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