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Cell Applications Inc Human Skeletal Muscle RNA: Total RNA prepared from human skeletal muscle tissue, 50 ug
Supplier: Cell Applications Inc 1H6050
Stringent quality control tests confirm RNA integrity and stability:Distinct 28S and 18S ribosomal RNA were shown on 1% denaturing gel before and after 37C incubationNo visible Genomic DNA band of 23 kb detectedNo genomic DNA contamination detected following PCR amplification using house-keeping gene G3PDH primersA 2.2 kb product from mRNA was detected following RT-PCR amplification using human heat shock gene Hsp60 primersA discrete band of 2 kb was detected upon hybridization with a human beta-actin cDNA probe in Northern Blot analysisPrimers were designed to detect mRNA for genes of interest to confirm cell phenotype
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