Acro Biosystems Recombinant Protein;Human TNF-alpha Protein, His Tag;HEK293;1MG;TNA,TNF,DIF,TNF-alpha,TNFalpha,TNFA,TNFSF2,TNF-A,cachexin,cachectin,TNF-α,TNFα
Manufacturer: Acro Biosystems TNAH52281MG
Recombinant Protein;Human TNF-alpha Protein, His Tag;1MG;Human TNF-alpha, His Tag (TNA-H5228) is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # NP_000585.2).;This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 18.2 kDa. The protein migrates as 18.2 kDa under reducing (R) condition (SDS-PAGE).;Immobilized Human TNF-alpha, His Tag (Cat. No. TNA-H5228) at 0.2 μg/mL (100 μl/well) can bind Human TNFR1, Fc Tag (Cat. No. TN1-H5251) with a linear range of 0.4-6.25 ng/mL (QC tested).;Humira (Adalimumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human TNF-alpha, His Tag (Cat. No. TNA-H5228) with an affinity constant of 0.244 nM as determined in a SPR assay (Biacore T200) (Routinely tested).;Human TNF-alpha, His Tag (Cat. No. TNA-H5228) induces cytotoxicity effect on the WEH1-13VAR cells in the presence of the metabolic inhibitor actinomycin D. The EC50 for this effect is 0.013-0.074 ng
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