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TOPOGEN INC Human Topoisomerase I
Supplier: TOPOGEN INC TG2005HRC3
High purity, catalytically active Human Topoisomerase I overexpressed in the baculovirus system and purified to single band homogeneity. These preparations are cheaper than native top1 made from human tissue and offer excellent purity and high specific activity. In addition, the 100 kDa form of recombinant topo I is stable and is less prone to proteolysis, relative to the native protein from human cells. The final preparation of Topo 1 is highly active in relaxation of plasmid DNA. Topo I as prepared and purified using the baculovirus overexpression system. Activity was determined by serial 2 fold dilutions using supercoiled DNA. One unit of Human Topo I can relax >50% of 0.25 μg (250 ng) of supercoiled plasmid DNA in 30 minutes at 37°C.
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