Sigma Aldrich Fine Chemicals Biosciences HuR-ARE Interaction Inhibi

Supplier:  Sigma Aldrich Fine Chemicals Biosciences 5383390001

A cell-permeable 2H-chromen-2-one based compound that competitively binds to Hu antigen R (HuR Ki 350 nM) and disrupts the interaction of HuR and adenine- and uridine-rich element (ARE) of target mRNAs. Displays dose-dependent binding to full-length HuR protein and to RRM1/2 fragment. Exhibits cytotoxicity towards tumor cells and reduces their viability (IC50 28.9 and 18.2 181M for HCT-116 and MioPaCa2 cells respectively). Has much reduced effect on normal WI-38 and CCD 841 CoN cells. Shown to induce cleavage of poly (ADP-ribose) polymerase and pro-caspase 3 (~ 50 181M) and reduce the half-life of Bcl-2 Msi1 and XIAP in HCT-116 cells (~20 181M) treated with actinomycin D (5 181g/ml).Please note that the molecular weight for this compound is batch-specific due to variable water content. Please refer to the vial label or the certificate of analysis for the batch-specific molecular weight. The molecular weight provided represents the baseline molecular weight without water.

Catalog No. 50-290-0861