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StemExpress Peripheral Blood Monocyte-Derived Immature Dendritic Cells, Frozen, 1.5 million/vial
Supplier: StemExpress PBIDC01.5C
Human peripheral blood monocyte-derived immature dendritic cells are derived from negatively selected monocytes. Non-monocytes are labeled and depleted from the peripheral blood mononuclear cell population using immunomagnetic particles leaving purified, untouched monocytes. Untouched monocytes are cultured for 4-5 days in culture medium containing 10% FBS plus GM-CSF and IL-4. After culture, cells are checked for the expression of CD11c, HLA-DR, and CD83 and the lack of CD14 expression by flow cytometry.
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