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Sigma Aldrich Fine Chemicals Biosciences Maleimide Activated KLH5MG
Supplier: Sigma Aldrich Fine Chemicals Biosciences K03835MG
Maleimide Activated KLH is provided in lyophilized ready to-use form eliminating the need for time-consuming activation and purification steps of the carrier protein. Maleimide Activated KLH contains approximately 160-320 maleimide groups for each KLH molecule. The conjugation reaction with a cysteine containing peptide is performed at pH 6.6-7.0. The extent of conjugation can then be determined by a colorimetric assay with Ellmans reagent to detect unreacted thiol groups in the cysteine-containing peptide.
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