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New England Biolabs, Inc. Mismatch Endonuclease I-4000 units
Supplier: New England Biolabs, Inc. M0678S
Mismatch Endonuclease I is a Mg2 dependent DNA endonuclease that specifically cleaves mismatched base pairs (TT GG and TG mismatches). Mismatch Endonuclease I cleaves the 3rd phosphodiester bond on the 5 side of the mismatched base in both strands leaving a 5 bp overhang. While Mismatch Endonuclease I prefers to cleave TT GG and TG DNA mismatches it will also readily cleave TI GI and GU DNA mismatches. Additionally Mismatch Endonuclease I has been shown to nick the thymine containing strand of TC DNA mismatches and cleave TG and TU (DNARNA) mismatches albeit to a lesser extent than DNADNA mismatches.
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