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Apexbio Technology LLC Mouse iPSC Chemical Reprogramming Cocktails Kit 500ml
Supplier: Apexbio Technology LLC K1022500
Mouse iPSC Chemical Reprogramming Cocktails Kit is designed specifically for the chemical induction of mouse somatic cells including mouse embryonic fibroblasts (MEFs) mouse neonatal fibroblasts (MNFs) mouse adult fibroblasts (MAFs) and adipose-derived stem cells (ADSCs) into chemically induced pluripotent stem cells (CiPSCs) The kit includes seven small-molecule compounds (VC6TFZ plus TTNPB) targeting multiple signaling pathways to initiate and stabilize the pluripotent state without genetic manipulation When combined sequentially with DZNep and subsequently cultured in 2i-conditioned medium (CHIR-99021 and PD0325901) this protocol can yield CiPSCs with reprogramming efficiency of approximately 0 2% Generated CiPSCs demonstrate embryonic stem cell-like morphology and pluripotency to differentiate into cell types from all three germ layers in vitro and in vivo suitable for regenerative medicine and developmental research
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