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ATCC ATCC CRL-10680 - Mouse L cells expressing human CDw32 (clone 16.2 CG7)
Supplier: ATCC CRL10680
Mouse L cells engineered to express human CDw32 (clone 16.2 CG7). These L-cell fibroblasts are commonly used as gamma-irradiated feeder cells for B cell culture and related immunology applications. Recommended culture conditions reported in literature include DMEM supplemented with 10% fetal bovine serum and 50 μg/mL gentamicin; incubate at 37°C.
- Stable expression of human CDw32 in an L-cell background.
- Suitable as feeder cells when gamma-irradiated.
- Grows in DMEM with 10% FBS.
- Reported antibiotic supplement: 50 μg/mL gentamicin.
- Can be cryopreserved after preparation for later use.
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