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ATCC pRE112 plasmid; in E. coli
Supplier: ATCC 87692
One of 4 allelic exchange suicide vectors (ATCC# 87691-87694) that provide both selection for chromosomal integration (ampR, cmLR, kanR or tetR) and counterselection for loss of vector DNA and the wild type allele.The construct is a suicide plasmid in any host not expressing pir. Cloned inserts may be integrated into the host chromosome (a single recombination event) following electroporation and appropriate antibiotic selection. - Gene 207: 149-157, 1998.The conditional R6K origin of replication requires that the pi protein be expressed in trans for plasmid maintenance.- Cell 15: 1199-1208, 1978Negative selection for sucrose sensitivity (sacB) selects for a second recombination event resulting in loss of vector DNA. The sacB1 allele is a variant of sacB with certain restriction sites removed by site directed mutagenesis. Expression of sacB confers sensitivity to sucrose. ? Mol. Microbiol. 18: 877-889, 1995.
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