Ubpbio NI-NTA AGAROSE, 5ML
Manufacturer: Ubpbio P30205
Protein Purification Reagents – Ni-NTA Resin (UBPBio catalog# P3020-5, P3020-25, P3020-100) – Ni-NTA XPure Agarose Resin can be used to purify 6xHis-tagged proteins expressed in series of expression vectors, such as E.coli., yeast, insect cells and mammalian cells. Ni-NTA XPure Agarose Resin consists of 90µm beads of highly cross-linked 6% agarose, to which Nitilotriacetic acid (NTA) has been coupled. The chelating group has then been charged with nickel ions (Ni2+). This form is very stable octahedral structure of nickel ions in the center, which can protect the nickel ions from attack of the competitive small molecule. The structure of Ni-NTA is compatible with a certain concentration of reducing agents, denaturing agents, detergents and other additives. Ni-NTA XPure Agarose Resin is highly stable and expand the range of suitable operating conditions.