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Electron Microscopy Sciences Nioprobe AFM SPM Calibration Specimen
Supplier: Electron Microscopy Sciences 80130NB
The Nioprobe device is used to determine the shape at the very apex of the tip probe for microscopy measurements.
The physical probe used in AFM imaging is not ideally sharp. As a consequence, an AFM image does not reflect the true sample topography impartially, but rather represents the interaction of the tip with the sample surface. There is no avoiding this imperfection, which sets real limits on what may be validly inferred from an AFM image.
Whether one is engaged in detailed, quantitative metrology or is simply using AFM images as a interpretive aid, it is imperative be able to assess these limits. The key here is to possess a reliable estimate of the sharpness of the tip apex. Reverse imaging of the probe is the most convenient means of obtaining the effective radius of the probe. For this purpose, the ideal characterization sample would consist of small, stiff, spiked features.
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