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Invent Biotechnologies Inc Minute™ Nuclear Proteasome Enrichment Kit - Spin Column-Based (20 preps)
Supplier: Invent Biotechnologies Inc PN041
The proteasome is a protein degradation system that requires metabolic energy and polymerization of ubiquitin that is different from lysosome-based protein degradation. The 26S proteasome is made up of two subcomplexes (a 20S proteasome and one or two19S regulatory particles). The proteasomes have been found mainly localized in cytosol but also detected in nucleus. To overcome these shortcomings, we have developed spin-column-based kits for enrichment of proteasome from nuclei (Cytosolic Proteasome Enrichment Kit is also available under Cat # PT-040). The protocol is simple and rapid with high yield. The gentle protocol maintains the association of proteasomes with ubiquitin and other proteins and is useful for the studies of proteasome structure and function. The highly enriched proteasomes can also be used as a starting material for affinity purifications.
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