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ATCC PBAD18KAN PLASMID IN E COLI
Supplier: ATCC 87396
One of several tightly controlled expression vectors (ATCC 87393 - ATCC 87402) regulated by the arabinose operon. Vectors differ in replicon, antibiotic resistance marker, multiple cloning sites and need for translation initiation sequences. Cultures should be grown in minimal media for more reproducible induction of expression. Expression is induced in glycerol containing media by addition of arabinose. Expression is repressed by addition of glucose or other catabolites. Cloned inserts must provide a translation initiation sequence (ATG) and ribosome binding site for expression. Plasmid contains bla (ampR) sequences surrounding the cmLR gene which could promote recombination if this plasmid is used in combination with other compatible ampR plasmids. Recombination can be avoided by the use of recA host strains, or it can be used to advantage to intentionally exchange markers among plasmids. The following primers can be used for sequencing of cloned inserts: 5' primer (27 - 8 bp
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