Sigma Aldrich Fine Chemicals Biosciences Phosphatase Alkaline from

Supplier:  Sigma Aldrich Fine Chemicals Biosciences P5521

The enzyme is a glycoprotein containing approximately 12% carbohydrate (6% hexoses and 6% other neutral sugars). Each molecule of alkaline phosphatase contains four zinc atoms and four disulfide bridges. Maximal activity with alkaline phosphatase is achieved in the presence of magnesium. It catalyzes the hydrolysis of phosphate monoesters such as p-nitrophenyl phosphate phenyl phosphate phenolphthalein phosphate glycerol phosphate -glycerol phosphate 2-phosphorylglycerate triosephosphate glucose-6-phosphate glucose 1-phosphate fructose 1-phosphate fructose 6-phosphate adenosine 5-phosphate adenosine 3-phosphate phosphoenolpyruvate and -nicotinamide adenine dinucleotide phosphate. Arsenate cysteine iodine inorganic phosphate pyrophosphate diisopropyl phosphate triphenylphosphate diisopropyl fluorophosphate and L-phenylalanine are some of the strong inhibitors of alkaline phosphatase.

Catalog No. 50-290-8120