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Alkali Scientific Phosphate Buffer #4 (0.1M,pH 4.5) 1000mL Bottle, 1/Pk

Supplier: Alkali Scientific MHHB02
Phosphate Buffer #4 is a prepared liquid reagent formulated to an acidic pH of 4.5 with a concentration of 0.1 M. It provides a stable low pH environment widely used in analytical chemistry, chromatography, and biological assays. The formulation relies on phosphate ion equilibrium, primarily the monobasic form (H2PO4 ), to maintain acidity and buffering capacity. In HPLC applications it is commonly used as a mobile phase component where pH 4.5 suppresses ionization of acidic analytes, improving retention and peak shape on reverse phase columns. It also supports enzymatic reactions that require acidic conditions, such as acid phosphatase activity. In pharmaceutical and laboratory testing it can simulate mildly acidic environments for dissolution, solubility studies, and routine analytical procedures.
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