Biotium PMA Real-Time PCR Bacterial Viability Kit - Mycobacterium tuberculosis (groEL2)

Supplier:  Biotium 31034

This PMA-PCR kit is designed for selective detection of viable bacteria from a specific strain using PMA dye and real-time PCR. This kit provides primers for specific amplification of Mycobacterium tuberculosis groEL2 gene. The kit has sufficient reagents for treating of 80 bacterial cultures and performing 200 PCR reactions with PMA. PMA is a high affinity photoreactive DNA binding dye that is weakly fluorescent by itself but becomes highly fluorescent upon binding to nucleic acids. The dye is cell membrane-impermeable and thus can be used to selectively modify DNA from dead cells with compromised membrane integrity, while leaving DNA from viable cells intact. PMA inhibits PCR amplification of modified DNA templates by a combination of removal of modified DNA during purification and inhibition of template amplification by DNA polymerases. Consequently the dye is useful in the selective detection of viable pathogenic cells by quantitative real-time PCR.

Catalog No. 50-196-4552