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Biotium Primary Antibody HL 60 Antigen Human Leukemia Antigen IPO-M6 CF740 1/EA
Supplier: Biotium BNC740228100
This antibody reacts with human leukemia cell line HL-60 and immunoprecipitates two proteins with MW of 48 kDa and 52 kDa It does not stain B-cell lines Daudi PHS Namalwa RPMI-1788 and T-cell lines CCRF-HSB2 Jurkat and Molt-4 This antibody stains monocytes and up to 10% of lymphocytes from peripheral blood of healthy donors Blast cells of patients with AMMonL (M5 following FAB classification) AMMonL (M4) and hairy cells leukemia are positive Its antigen is particularly expressed on blood cells from patients with infectious mononucleosis and CLL Histiocytes and macrophages are also positive Malignant cells from patients with AML (M1 and M2) T-ALL B-ALL are not stained HL-60 cells are used as an in vitro model of acute promyelocytic leukaemia and for differentiation and apoptosis studies The HL60 cell line was established in 1977 from a patient with acute myeloid leukaemia The cells largely resemble promyelocytes but can be induced to differentiate terminally in vitro Some reagents
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